(1) Preparation room
In the preparation room, various facilities and equipment are available to facilitate the process of preparing and processing mediums and samples. Among the essential features are reagent cabinets, which provide secure storage for various reagents and chemicals. Additionally, there are spacious counters where utensils and materials can be conveniently kept. The room also accommodates experimental platforms for conducting different experiments and electric furnaces for carrying out heating processes. For the preservation of sensitive samples, refrigerators are present. Furthermore, drains are available to ensure proper disposal of waste materials. Lastly, power supplies are easily accessible to support the smooth functioning of equipment in the preparation room.
(2) cleaning room
It is important to have a dedicated cleaning room for washing utensils and other kitchen equipment as they can become contaminated with harmful microorganisms. To ensure the space is well-equipped, heaters, cooking pots, washing utensils, buckets and a variety of cleaning agents such as bottle brushes, detergent powder, soap, and washing powder should be provided. By doing so, we can effectively clean and sanitize our utensils to prevent any potential foodborne illnesses from developing.
(3) Sterilization room
The sterilization room serves the purpose of eliminating pathogenic microorganisms, like bacteria, from culture medium and various medical instruments through the process of sterilization. To effectively carry out this task, the room is equipped with sterilization equipment such as high pressure steam sterilizers and ovens. These facilities work to ensure that the unwanted microorganisms are effectively destroyed, and the sterile equipment or medium is safe for use.
(4) aseptic room
The asepsis room, also known as the inoculation room, is a specialized laboratory designed for aseptic operations such as bacterial purification and systematic inoculation. The process of inoculation and transplantation of strains is of paramount importance in microbiology, since it involves the introduction of pure strains to minimize contamination by extraneous bacteria. In the general environment, the presence of dust and diverse bacteria presents a major challenge, as it can result in pollution and interfere with the inoculation process. As a result, the asepsis room is a vital resource for maintaining a contamination-free environment for successful microbial operations.
(5) Constant temperature culture room
1. Setting of the culture room
The culture room is designed to consist of two parts, an indoor and an outdoor area. The cabinet room serves as the main culture room, while the outer room acts as a buffer space. To facilitate air sterilization, it is important that the room volume is not too large. The cabinet room has an area of about 14m2 (3.2×4.4) and the outer room has an area of about 6m2 (3.2×1.8). Both rooms have a height of approximately 2.5m and are constructed using Tianhua board.
To ensure proper ventilation, it is important to have a vent with an air filter device installed on the upper part of the wall that separates the cabinet room and the outer room.
Constant temperature and humidity machines should be installed to cater to the temperature requirements of microorganisms. This installation is essential to meet their needs effectively and ensure their optimal growth and survival.
To ensure thorough sterilization of the room, it is recommended to install a UV lamp in the center of both the inside and outside areas. This will effectively eliminate any harmful bacteria and viruses that may be present in the environment. It is important to follow this guideline in order to maintain a safe and hygienic space for all occupants.
2. Cultivate indoor equipment and appliances
The cabinet room is typically furnished with a culture rack and a bottle shaking machine, also known as a shaking bed. Shake bottle machines commonly come in two types: rotary and reciprocating.
In the outer room, we need to provide a range of safety equipment and supplies. These should include special clothing, such as suits and hats, as well as masks to protect the workers. Additionally, hand-held sprayers are essential for applying various solutions. It is important to have a 5% coal acid solution readily available for specific tasks. Moreover, cotton balls soaked in 70% alcohol are crucial for disinfecting and cleansing purposes. Ensuring that all these items are easily accessible and well-stocked is essential for maintaining a safe and efficient working environment in the outer room.
3. Sterilization and disinfection of culture room
The aseptic room undergoes sterilization and disinfection measures to maintain its cleanliness and prevent the spread of contaminants. These measures are essential for creating a sterile environment.
To begin with, sterilization procedures are implemented in the aseptic room. This involves using methods such as heat or chemicals to eliminate all forms of microorganisms, including bacteria, viruses, and fungi. Heat sterilization can be achieved through techniques like autoclaving, where items are subjected to high temperatures and pressure. Chemical sterilization, on the other hand, utilizes substances like hydrogen peroxide or ethylene oxide to kill off any microorganisms present.
In addition to sterilization, disinfection is also carried out within the aseptic room. Disinfection refers to the eradication of potentially harmful microorganisms to a level that is considered safe. Various disinfectants are available for this purpose, such as bleach, quaternary ammonium compounds, or alcohol-based solutions. Surfaces, equipment, and tools used in the aseptic room are thoroughly disinfected to ensure the elimination of any pathogens that may be present.
To maintain the sterilized and disinfected environment, strict protocols and practices are followed. These include the use of aseptic techniques by personnel working in the room, such as wearing gloves, masks, and gowns to prevent contamination. Regular monitoring and testing are also conducted to ensure the effectiveness of the sterilization and disinfection measures.
Overall, the sterilization and disinfection measures implemented in the aseptic room play a crucial role in creating and maintaining a sterile environment. These measures help prevent the introduction and transmission of microorganisms, ensuring that the room remains free from contaminants that could compromise its intended purpose.
It is not suitable to place a small range of culture in a constant temperature incubator; however, it can be placed and maintained in a constant temperature incubator.
(6) General laboratories
This is a facility dedicated to conducting observation, counting, and physiological and biochemical analysis of microorganisms. The room's furnishings vary considerably based on the specific focus of the research. Typically, you will find a laboratory bench, micromicrobiology bulletin, cabinet, and stool. The experimental platform must be smooth and even, while the cabinet should provide sufficient space for commonly used equipment and medications.
(7) Other requirements of the laboratory
To ensure that the laboratory functions smoothly, it is essential that the water, electricity, and gas systems are designed to meet its specific needs. This includes considering the capacity, layout, and performance of these utilities. The capacity of the water supply should be sufficient to meet the demands of the laboratory, taking into account factors such as the number of sinks, faucets, and other water-dependent equipment. Similarly, the electrical system should be designed to handle the power requirements of the laboratory's equipment, ensuring that there are enough outlets and circuits to support their operation. Lastly, the gas system should be able to provide the necessary fuel for any gas-powered devices or experiments within the laboratory. It is important to carefully plan and layout these systems to optimize their performance, ensuring efficient and effective functioning of the laboratory.